Hóa chất nuôi cấy mô, tế bào động vật


STT Code Mô tả hàng hóa Hãng sx SL Đóng gói
1
Môi trường nuôi cấy



11885084 (D-MEM) (1X), liquid (low glucose), 500ml Invitrogen 1 500ml

10313021 (D-MEM) (1X), liquid (high glucose), 500ml Invitrogen 1 500ml

11380-037 Minimum Essential Medium (MEM) (1X), liquid, 500ml Invitrogen 1 500ml

22400089 RPMI Medium 1640 (1X), liquid, 500ml Invitrogen 1 500ml
2
Huyết thanh bổ sung



10082-147 Fetal Bovine Serum, Certified, Heat-Inactivated, 500ml Invitrogen 1 500ml
3
Dung dịch muối đệm (rửa)



14200-166 Phosphate buffer saline, 10X, 500ml Invitrogen 1 500ml
4
Buffers bổ sung vào môi trường



15630080 HEPES Buffer Solution (1 M) Invitrogen 1

25080-094 Sodium Bicarbonate, 7,5%(w/v), 100ml Invitrogen 1 100ml
5
Hóa chất phân tách tế bào



25200-056 Trypsin, 0.25% (1X) with EDTA 4Na, liquid, 100ml Invitrogen 1 100ml

T4174-100ML Trypsin-EDTA solution - 10X Sigma 1 100ml

17100-017  Collagenase Type I (use with cells from these tissues: Epithelial, Lung, Fat, Adrenal) Invitrogen 1 1g

17101-015 Collagenase Type II (use with cells from these tissues: Liver, Bone, Thyroid, Heart, Salivary gland) Invitrogen 1 1g

17104-019 Collagenase Type IV (use with tissues from pancreas and isolation of pancreatic islets) Invitrogen 1 1g
6
Các chất bổ sung



25030-149 L-Glutamin, 200mM (100X), liquid Invitrogen 1 20ml

15023-021 D-Glucose (Dextrose) Invitrogen 1 1kg

11130-051  MEM Amino Acids Solution (50X), liquid Invitrogen 1 100ml

11360-070  MEM Sodium Pyruvate Solution 10mM (100X), liquid Invitrogen 1 100ml

11140-050 MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid Invitrogen 1 100ml

11120-052 MEM Vitamin Solution (100X), liquid Invitrogen 1 100ml

21985-023  2-Mercaptoethanol (1,000X), liquid Invitrogen 1 50ml
7
Kháng sinh



15140-122 Penicillin-Streptomycin, liquid Invitrogen 1

15640-055 Penicillin-Streptomycin-Neomycin , liquid Invitrogen 1 100ml

21810-031 Neomycin Sulfate, powder Invitrogen 1 100g

15240-062 Antibiotic-Antimycitic (100X), liquid Invitrogen 1 100ml

15710-064 Gentamycin solution (10mg/mL) Invitrogen 1 10ml
8
Kháng sinh chọn lọc



10131-027 Geneticin Selective Antibiotic, liquid Invitrogen 1 20ml

10687-010 Hygromycin B, liquid Invitrogen 1 20ml

A11138-03 Puromycin Dihydrochloride, liquid Invitrogen 1 10 lọ x1ml

R250-01 Zeocin Selective Antibiotic, powder Invitrogen 1 1g
9
Bộ kit xác định nhiễm khuẩn môi trường nuôi cấy



C-7028 Cell culture Contamination Detection kit Invitrogen 1 200 assays

M7006 MycoFluor Mycoplasma Detection kit Invitrogen 1 1 kit
10
Attachment factors and matrices



33016-015 Human plasma Fibronectin Invitrogen 1 5mg

23017-015 Natural Mouse lamini Invitrogen 1 1mg
11
Instrument



C10227 Countess Automated Cell Counter (includes 50 Countess cell counting chamber slides and 2ml of Trypan Blue Stain) Invitrogen 1 1unit

C10228 Chamber Slides 50 slides (100 counts) Invitrogen 1 1 box

99503 Mallassez Hemacytometer Invitrogen 1 1 unit

MPK5000 Neon Transfection Systems (For transfecting mammalian cells, including primary and
stem cells, with high transfection efficiency)
Invitrogen 1 1 unit

Q32866 Qubit 2.0 Fluorometer (thế hệ 2) (ideal for cloning, sequencing, transfection, qPCR, microarrays, protein gels, Western blots, and protein activity assays.) Invitrogen 1 1 unit
12
Hóa chất chuyển genn vào tế bào



11668-027 Lipofectamine 2000 Invitrogen 1 0.75ml

111668-019 Lipofectamine 2000 Invitrogen 1 1.5ml

13778-150 Lipofectamine RNAiMAX (provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments) Invitrogen 1 1.5ml

13778-075 Lipofectamine RNAiMAX (provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments) Invitrogen 1 0.75ml
13
Hóa chất nghiên cứu DNA gắn huỳnh quang



A-20191 Alexa Fluor® 488 Oligonucleotide Amine Labeling Kit *3 labelings* (Oligonucleotides labeled with this kit can be used as primers and as probes in hybridization experiments but should not be used for automated DNA sequencing since the Alexa Fluor dye in the kit is supplied as mixed isomers) Invitrogen 1 1 kit

U-21650 Ulysis™ Alexa Fluor® 488 Nucleic Acid Labeling Kit (with our high-quality, green-fluorescent Alexa Fluor 488 dye – the result is a simple, direct method for labeling samples for hybridization experiments) Invitrogen 1 1 kit

U-21660 Ulysis™ Alexa Fluor® 647 Nucleic Acid Labeling Kit (with our high-quality, green-fluorescent Alexa Fluor 647 dye – the result is a simple, direct method for labeling samples for hybridization experiments) Invitrogen 1 1 kit

    A-21665  ARES™ Alexa Fluor® 488 DNA Labeling Kit *5-10 labelings* (The labeled probes can be used for fluorescence in situ hybridization (FISH) and microarray techniques) Invitrogen 1 1 kit

    A10468  APEX™ Alexa Fluor® 488 Antibody Labeling Kit


14
Hóa chất nghiên cứu miễn dịch huỳnh quang



PV3612 Recombinant Aurora kinase A Invitrogen 1

PV3970 Recombinant Aurora kinase B Invitrogen 1

36-5200 Aurora B antibody Invitrogen 1

37-2000 Survivin, Mouse monoclonal antibody Invitrogen 1

A11126 Alpha tubulin antibody Invitrogen 1


Kháng thể thứ 2



A11001 Alexa Fluor® 488 goat anti-mouse IgG (H+L) *2 mg⁄mL Invitrogen 1 0.5ml

A11003 Alexa Fluor® 546 goat anti-mouse IgG (H+L) *2 mg⁄mL Invitrogen 1 0.5ml

A11008 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) *2 mg⁄mL Invitrogen 1 0.5ml

A11010 Alexa Fluor® 546 goat anti-rabbit IgG (H+L) *2 mg⁄mL Invitrogen 1 0.5ml

A-10235  Alexa Fluor® 488 Protein Labeling Kit *3 labelings* Invitrogen 1


Alexa Fluor® Fluorescent Streptavidin Conjugates



    A-10235  Alexa Fluor® Fluorescent Streptavidin Conjugates (this kit can be used to make conjugates of other proteins larger than ~40,000 daltons. The kit provides sufficient material to perform three protein conjugations and purifications) Invitrogen 1 1 kit

S11223 S32354 Alexa Fluor® 488 Streptavidin (S11223 (1 mg), S32354 (0.5 mL) Invitrogen 1 1 kit

S21378 S32358 Alexa Fluor® 680 Streptavidin (S21378 (1 mg), S32358 (0.5 mL) Invitrogen 1 1 kit


Alexa Fluor® Reactive Dyes



A-20501MP  Alexa Fluor® 555 hydrazide, tris(triethylammonium) salt (This orange-fluorescent polar dye can be loaded into cells by microinjection, infusion from patch pipette or uptake induced by our Influx pinocytic cell-loading reagent) Invitrogen 1 1mg

A30676 Alexa Fluor® 488 cadaverine, sodium salt Invitrogen 1 1mg

MHCIFG20  IFN-γ, Mouse Anti-Human, (Alexa Fluor® 488) Invitrogen 1 0.5ml
15
Hóa chất sinh học phân tử



15553-027 Ultra Pure™ 10% SDS Solution Invitrogen 1 4x100ml

93362-250G Trizma® base (Tris base)
BioUltra, for molecular biology, ≥99.8% (T)
Sigma 1 250g

15506-017 UltraPure™ Tris Hydrochloride (Tris-HCl) Invitrogen 1 500g

92560-10G Polyacrylamide Sigma 1 10g

PV3227 ATP, 10mM Invitrogen 1     500 µl

15585-011 UltraPure™ 10 mg⁄ml Ethidium Bromide Invitrogen 1

15504020 TRIS 1KG Invitrogen 1

P2325 DTT Invitrogen 1

19524-016  Biotin-14-dATP (can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase) Invitrogen 1 50nmol

19518-018 Biotin-14-dCTP (This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP (1), or labeling DNA with terminal deoxynucleotide transferase (2) or random primers synthesis) Invitrogen 1 50nmol

10297-018 100 mM dNTP Set Invitrogen 1

18427-013 10 mM dNTP Mix Invitrogen 1

P-3456 Taxol Invitrogen 1

15524010 TEMED 30ML Invitrogen 1

15527013 GLYCINE 500G Invitrogen 1

15525017 SODIUM DODECYL SULFATE 500G Invitrogen 1

D9307-50UN JumpStart™ Taq DNA Polymerase
with MgCl2; Code: D9307-50UN/without MgCl2; (code: D4184-50UN; giá tương tự)
Sigma 1 50UN

D4545-50UN Taq DNA Polymerase from Thermus aquaticus
with 10× reaction buffer without MgCl2
Sigma 1 50UN


Probes



A192P.0100 Human Papillomavirus Type 16⁄18 Probe Invitrogen 1 0.5ml

  A500P.0100  Biotinylated Oligonucleotides for EBER products Invitrogen 1 0.5ml

    84-1300  SPoT-Light EGFR Probe Invitrogen 1 0.4ml


Fluorescently Labeled Nucleotides



    A32770  Alexa Fluor® 555 -aha-dCTP *1 mM in TE buffer* Invitrogen 1 50ul


Alexa Fluor® 647 -aha-dCTP *1 mM in TE buffer* Invitrogen 1 50ul

G1393-20ML Gelatin solution
2% in H2O, tissue culture grade, BioReagent, sterile, Type B, suitable for cell culture
Sigma 1 20ml

D8418-50ML Dimethyl sulfoxide (DMSO)
BioReagent, for molecular biology, ≥99.9%
Sigma 1 50ml

F3651-100MG Fluorescein 5(6)-isothiocyanate (FITC)
~90% (HPLC)
Sigma 1 100mg

FITC1-1KT FluoroTag™ FITC Conjugation Kit Sigma 1 1kit


TaqMan® Gene Expression Assays Invitrogen 1


TaqMan® MicroRNA Assays Invitrogen 1


TaqMan® SNP Genotyping Assays Invitrogen 1


TaqMan® Copy Number Assays Invitrogen 1